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Journal / Genetics / QTL定位和CRISPR /…
JoVE Journal
Genetics

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JoVE Journal Genetics
QTL定位和CRISPR / Cas9编辑识别抗药性基因
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QTL定位和CRISPR / Cas9编辑识别抗药性基因

Article DOI: 10.3791/55185-v • 11:37 min • June 22nd, 2017
June 22nd, 2017 •
1, 2, 2
1State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, 2Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University

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详细介绍了如何使用基于全基因组序列的遗传图谱进行QTL定位，以鉴定弓形虫中的药物抗性基因，以及如何使用有效编辑基因组靶标的CRISPR / Cas9系统进行验证，在这种情况下耐药基因。

Tags

遗传学，第124期，定量性状基因座（QTL）测绘，聚类定期间隔短回文重复序列（CRISPR），指导RNA（gRNA），正丁芬素，单核苷酸多态性（SNP），遗传交叉，全基因组测序（WGS）
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