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JoVE Journal Developmental Biology
Instrueret differentiering af Primitive og endelige hæmatopoietisk ophav fra humane pluripotente stamceller
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Instrueret differentiering af Primitive og endelige hæmatopoietisk ophav fra humane pluripotente stamceller

Article DOI: 10.3791/55196-v • 14:37 min • November 1st, 2017
November 1st, 2017 •
1, 1
1Department of Internal Medicine, Hematology Division, Washington University in St. Louis

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Vi præsenterer her, humane pluripotente stamceller (hPSC) kultur protokoller, bruges til at differentiere hPSCs i CD34+ hæmatopoietisk stamfaderen. Denne metode bruger fase-specifikke manipulation af canonical WNT signalering til at angive celler udelukkende til enten det endelige eller primitive hæmatopoietisk program.

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Udviklingsmæssige biologi sag 129 pluripotente stamceller humane embryonale stamceller cellekultur embryoid organer WNT signalering endelige bloddannelsen primitive bloddannelsen hemogenic endotelet
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