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Immunology and Infection

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JoVE Journal Immunology and Infection
Una fluorescenza a base di linfociti Assay Adatto per high-throughput screening di piccole molecole
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Una fluorescenza a base di linfociti Assay Adatto per high-throughput screening di piccole molecole

Article DOI: 10.3791/55199-v • 08:43 min • March 10th, 2017
March 10th, 2017 •
1, 2, 2, 2, 1,2,3
1Department of Microbiology, Infectiology and Immunology, Université de Montréal, 2Department of Pharmacology and Physiology, Université de Montréal, 3Molecular Biology Program, Université de Montréal

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Presentiamo in questo studio un test basato sulla fluorescenza romanzo utilizzando linfociti derivati ​​da un topo transgenico. Questo test è adatto per high-throughput screening (HTS) di piccole molecole dotate di capacità di una inibizione o la promozione di attivazione dei linfociti.

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Immunologia lo screening high-throughput screening fenotipico lo screening basata sul target scoperta di nuovi farmaci i linfociti le cellule T cellule B fluorescenza proteina fluorescente verde Nur77 immunosoppressione l'attivazione
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