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Journal / Developmental Biology / Isolering og karakterisering av Mesenchymale…
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Developmental Biology

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JoVE Journal Developmental Biology
Isolering og karakterisering av Mesenchymale stromale celler fra human navlestreng og Fetal Placenta
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Isolering og karakterisering av Mesenchymale stromale celler fra human navlestreng og Fetal Placenta

Article DOI: 10.3791/55224-v • 07:06 min • April 3rd, 2017
April 3rd, 2017 •
1,2, 1,2, 1,2, 3, 1,2, 2,4, 1,2
1Department of Biological Sciences, Oakland University, 2OU-WB Institute for Stem Cell and Regenerative Medicine, 3Department of Obstetrics and Gynecology, St. John Provindence - Providence Park Hospital, 4Department of Neurosurgery, Beaumont Health System

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Her presenterer vi en protokoll for disseksjon av human navlestrengen (UC) og føtalt placenta prøven i ledningen foring (CL), Wharton gelé (WJ), ledning-placenta knutepunkt (CPJ), og føtal placenta (FP) for isolering og karakterisering av mesenchymale celler (stromale MSCene) ved hjelp av et utenforliggende kulturteknikk.

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Developmental Biology perinatal navlestreng Cord-placenta Junction placenta Mesenchymale stromale celler MSC markører multipotente celler Pluripotent markører
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