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Journal / Developmental Biology / Isolamento e caratterizzazione di cellule…
JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology
Isolamento e caratterizzazione di cellule mesenchimali stromali dal Cordone ombelicale e placenta fetale
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Isolamento e caratterizzazione di cellule mesenchimali stromali dal Cordone ombelicale e placenta fetale

Article DOI: 10.3791/55224-v • 07:06 min • April 3rd, 2017
April 3rd, 2017 •
1,2, 1,2, 1,2, 3, 1,2, 2,4, 1,2
1Department of Biological Sciences, Oakland University, 2OU-WB Institute for Stem Cell and Regenerative Medicine, 3Department of Obstetrics and Gynecology, St. John Provindence - Providence Park Hospital, 4Department of Neurosurgery, Beaumont Health System

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Qui, presentiamo un protocollo per la dissezione di cordone umano ombelicale (UC) e il campione placenta fetale in cavo rivestimento (CL), gelatina di Wharton (WJ), cavo-placenta giunzione (CPJ), e placenta fetale (FP) per l'isolamento e caratterizzazione di cellule stromali mesenchimali (MSC) utilizzando la tecnica di espianto.

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Biologia evolutiva perinatali cordone ombelicale giunzione Cord-placenta placenta cellule stromali mesenchimali marcatori MSC cellule multipotenti marcatori Pluripotenti
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