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JoVE Journal Neuroscience
Enkeltcelle RNA-Seq av definerte subsets av retinal Ganglion-celler
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Enkeltcelle RNA-Seq av definerte subsets av retinal Ganglion-celler

Article DOI: 10.3791/55229-v • 11:26 min • May 22nd, 2017
May 22nd, 2017 •
1, 2, 2, 1, 2
1Department of Genetics, Development, and Cell Biology, Iowa State University, 2Department of Neurobiology, Northwestern University

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Her presenterer vi en kombinatorisk tilnærming for klassifisering av nevrale celletyper før isolasjon og for den etterfølgende karakterisering av enkeltcelletransskriptomer. Denne protokollen optimaliserer utarbeidelsen av prøver for vellykket RNA-sekvensering (RNA-Seq) og beskriver en metodikk som er spesielt utviklet for å forbedre forståelsen av cellulært mangfold.

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Neuroscience Intrinsically lysfølsomme retinal ganglion celler elektrofysiology RNA sekvensering single-cell isolasjon lav inngang RNA sekvensering neuroner
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