Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / Rapid Deletion Production in Fungi via
JoVE Journal
Genetics

A subscription to JoVE is required to view this content.

JoVE Journal Genetics
Rapid Deletion Production in Fungi via
Generation of Fluorescent Protein Fusions in Candida Species
Journal (Genetics)
Generation of Fluorescent Protein Fusions in Candida Species
Producing Gene Deletions in Escherichia coli by P1 Transduction with Excisable Antibiotic Resistance Cassettes
Journal (Genetics)
Producing Gene Deletions in Escherichia coli by P1 Transduction with Excisable Antibiotic Resistance Cassettes
 
Click here for the English version

Rapid Deletion Production in Fungi via

Article DOI: 10.3791/55239-v • 11:24 min • June 12th, 2017
June 12th, 2017 •
1, 2, 3, 1
1Toxicology and Mycotoxin Research Unit, NPRC, USDA-ARS, 2Hazera Seeds LTD, Brurim, 3Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora” - Universidad de Málaga - Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Estación Experimental “La Mayora”

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Gendeletionsmutanter som alstras genom homolog rekombination är guldstandarden för genfunktionsstudier. OSCAR (One Step Construction av Agrobacterium-Recombination-ready-plasmids) -metoden för snabb generering av deletionskonstruktioner beskrivs. Agrobacterium- medierad svamptransformation följer. Slutligen presenteras en PCR-baserad bekräftelsemetod av gendeletioner i svamptransformanter.

Tags

Genetik Utgåva 124 Svamptransformation Svampdeletionsmutantgenerering ATMT Enstegsbyggande av
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter