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Journal / Developmental Biology / Analys av kromosomsegregation, histonacetylering…
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Developmental Biology

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JoVE Journal Developmental Biology
Analys av kromosomsegregation, histonacetylering och spindelmorfologi i hästocyter
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Analys av kromosomsegregation, histonacetylering och spindelmorfologi i hästocyter

Article DOI: 10.3791/55242-v • 12:11 min • May 11th, 2017
May 11th, 2017 •
1, 1,2, 3, 3, 4, 5, 3, 6, 1, 1, 3
1Department of Health, Animal Science and Food Safety, University of Milan, 2IRCCS. Istituto Ortopedico Galeazzi, 3PRC, CNRS, IFCE, Université de Tours, INRA, 4PAO, INRA, 5Clinique des Animaux de Compagnie et des Équidés, Université de Liège, 6University of Agricultural Sciences and Veterinary Medicine, Cluj-Napoca, Romania

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Detta manuskript beskriver ett experimentellt tillvägagångssätt för att morfologiskt och biokemiskt karakterisera hästocyter. Specifikt illustrerar föreliggande arbete hur man samlar omogena och mogna hästocyter genom ultraljudstyrd äggupphämtning (OPU) och hur man undersöker kromosomsegregation, spindelmorfologi, global histonacetylering och mRNA-uttryck.

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Utvecklingsbiologi utgåva 123 aneuploidy oocytmognad metafas meios epigenetisk histon-acetyltransferas histon-deacetylas assisterad reproduktion äggupptagning
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