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Journal / Genetics / Mappatura delle interazioni RNA-RNA globalmente…
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JoVE Journal Genetics
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Mappatura delle interazioni RNA-RNA globalmente utilizzando Psoralen biotinilato

Article DOI: 10.3791/55255-v • 11:32 min • May 24th, 2017
May 24th, 2017 •
1, 2, 2, 1
1Stem Cell and Regenerative Biology, Genome Institute of Singapore, A*STAR, 2Computational and Systems Biology, Genome Institute of Singapore, A*STAR

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Qui, abbiamo dettagliato il metodo di equilibrio di P soralen crosslinked, L igated e S elected H ybrids (SPLASH), che consente mapping a livello genomico delle interazioni RNA-RNA intramolecolari e intermolecolari in vivo . SPLASH può essere applicato per studiare interagenti RNA di organismi compresi lieviti, batteri e umani.

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Genetica Numero 123 RNA genomica interazione sequenza struttura umana
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