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Immunology and Infection

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JoVE Journal Immunology and Infection
Intracellular Staining and Flow Cytometry to Identify Lymphocyte Subsets within Murine Aorta, Kidney and Lymph Nodes in a Model of Hypertension
Microfluidic Co-Culture Models for Dissecting the Immune Response in in vitro Tumor Microenvironments
Journal (Immunology and Infection)
Microfluidic Co-Culture Models for Dissecting the Immune Response in in vitro Tumor Microenvironments
Isolation and Flow Cytometric Characterization of Murine Small Intestinal Lymphocytes
Journal (Immunology and Infection)
Isolation and Flow Cytometric Characterization of Murine Small Intestinal Lymphocytes
 

Intracellular Staining and Flow Cytometry to Identify Lymphocyte Subsets within Murine Aorta, Kidney and Lymph Nodes in a Model of Hypertension

Article DOI: 10.3791/55266-v • 09:20 min • January 28th, 2017
January 28th, 2017 •
1, 2, 3, 1
1Department of Medicine, Division of Clinical Pharmacology, Vanderbilt University Medical Center, 2Department of Molecular Physiology and Biophysics, Vanderbilt University, 3Department of Pharmacology and Toxicology, Faculty of Pharmacy, Mansoura University

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Summary
Automatic Translation

This article provides detailed methodology to identify and quantify functional T lymphocyte subsets present within murine kidney, aorta and lymph nodes by intracellular staining and flow cytometry. The model of angiotensin II induced hypertension was chosen to explain, step-by-step, the procedures and fundamental principles of flow cytometry and intracellular staining.

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Intracellular Staining Flow Cytometry Lymphocyte Subsets Murine Aorta Kidney Lymph Nodes Hypertension Model Cardiovascular Disease Single-cell Suspensions Immune Cells Angiotensin II-induced Hypertension Identification Quantification Solid Organs Disinfect Mouse Chest Perfuse Vasculature Cold PBS Blanched Organs Abdominal Aortic-draining Lymph Nodes Dissecting Microscope Kidneys Thoracic And Abdominal Aorta
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