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Journal / Biology / Methoden om te studeren epitheliale Transport…
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JoVE Journal Biology
Methoden om te studeren epitheliale Transport Protein functie en de expressie in Inheemse darm en Caco-2-cellen gekweekt in 3D
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Methoden om te studeren epitheliale Transport Protein functie en de expressie in Inheemse darm en Caco-2-cellen gekweekt in 3D

Article DOI: 10.3791/55304-v • 11:27 min • March 16th, 2017
March 16th, 2017 •
*1, *1, 1, 1, 1, 1,2, 1,2, 1,2, 1
1Department of Medicine, University of Illinois at Chicago, 2Department of Research, Jesse Brown VA Medical Center
* These authors contributed equally

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We beschrijven eenvoudige methoden de regulering van intestinale serotonine transporter (SERT) functie en expressie onder toepassing van een in vitro celkweek model van Caco-2-cellen gekweekt in 3D en een ex vivo model van muis darmen bestuderen. Deze methoden zijn geschikt voor de studie van andere epitheliale transporters.

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Cellular Biology transportfunctie strippen slijmvlies Ussing Kamer 3D Caco-2 kleuring van 3D-cellen serotonine transporter SERT
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