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JoVE Journal
Developmental Biology
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Solea Senegalensis精子中活性氧的细胞内定位评价
 
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Solea Senegalensis精子中活性氧的细胞内定位评价

Article DOI: 10.3791/55323-v 10:24 min March 11th, 2018
March 11th, 2018

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Summary

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此协议描述了一个用于检测Solea senegalensis精子中的 H2O2本地化的详细方法, 使用敏感的 fluorochrome DCFH-DA 为 ROS、线粒体的活线粒体染色和细胞核的 DAPI可视化, 分别。该协议的设计是在2小时内进行, 无论是新鲜或解冻精子。

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发育生物学 问题 133 Solea Senegalensis 精子 线粒体 超低温保存 线粒体染色 DCFH-DA DAPI 共焦显微术
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