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Journal / Genetics / Dannelse af fluorescerende protein-fusioner i
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Dannelse af fluorescerende protein-fusioner i
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Dannelse af fluorescerende protein-fusioner i

Article DOI: 10.3791/55333-v • 09:27 min • March 4th, 2017
March 4th, 2017 •
1, 2, 1
1Department of Pediatrics, University of Minnesota, 2Department of Molecular Microbiology and Biotechnology, Tel Aviv University

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PCR-medieret gen modifikation kan anvendes til at danne fluorescerende protein fusioner i Candida arter, hvilket letter visualisering og kvantificering af gærceller og proteiner. Heri præsenterer vi en strategi for at konstruere en fluorescerende protein fusion (Eno1-FP) i Candida parapsilosis.

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Genetik , PCR-medieret gen ændring fluorescerende protein, Lithium acetat transformation,
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