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Journal / Genetics / Die Erzeugung von Fluorescent Protein Fusions in
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JoVE Journal Genetics
Die Erzeugung von Fluorescent Protein Fusions in
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Die Erzeugung von Fluorescent Protein Fusions in

Article DOI: 10.3791/55333-v • 09:27 min • March 4th, 2017
March 4th, 2017 •
1, 2, 1
1Department of Pediatrics, University of Minnesota, 2Department of Molecular Microbiology and Biotechnology, Tel Aviv University

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PCR-vermittelte Gen - Modifikation kann verwendet werden , fluoreszierendes Protein - Fusionen in Candida - Spezies zu erzeugen, die Visualisierung und Quantifizierung von Hefezellen und Proteinen erleichtert. Hier präsentieren wir eine Strategie für ein fluoreszierendes Protein - Fusion (ENO1-FP) in Candida parapsilosis zu konstruieren.

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Genetik Ausgabe 121, PCR-vermittelte Genveränderung Fluorescent Protein, Lithiumacetat-Transformation,
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