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JoVE Journal Developmental Biology
Culture of Adult Transgene Sebrafisk Netthinne explants for Live-celle Imaging av multiphoton Mikros
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Culture of Adult Transgene Sebrafisk Netthinne explants for Live-celle Imaging av multiphoton Mikros

Article DOI: 10.3791/55335-v • 11:55 min • February 24th, 2017
February 24th, 2017 •
1, 1, 1,2, 1
1Department of Biological Sciences, University of Notre Dame, 2Department of Neurosurgery, Mayo Clinic

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Sebrafisk retinal regenerasjon har for det meste blitt undersøkt ved hjelp av faste netthinner. Men dynamiske prosesser som interkinetic atom migrasjon oppstår under regenerativ respons og krever levende celle bildebehandling for å undersøke de underliggende mekanismene. Her beskriver vi kultur og bilde forhold til å overvåke Interkinetic Nuclear Migration (INM) i sanntid ved hjelp multiphoton mikroskopi.

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Developmental Biology retinal explant multiphoton mikroskopi live-cell imaging sebrafisk interkinetic atom migrasjon kultur gjenfødelse Müller gliaceller mitose hastighet
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