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Biochemistry

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JoVE Journal Biochemistry
Multimer-PAGE
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Journal (Biochemistry)
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Journal (Biochemistry)
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Multimer-PAGE: En metode for å fange og løse protein komplekser i biologiske prøver

Article DOI: 10.3791/55341-v • 07:40 min • May 5th, 2017
May 5th, 2017 •
1, 2, 3, 3
1Physiology, Michigan State University, 2Center for Neurodegenerative Science Van Andel Institute, 3Pharmaceutical Sciences, Wayne State University

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En fremgangsmåte for å stabilisere og å separere naturlig forekommende protein komplekser fra umodifisert vev lysat ved anvendelse av et amin-reaktivt protein tverrbinder koplet til en ny to-dimensjonal polyakrylamid gelelektroforese (PAGE) system er presentert.

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Biochemistry utgave 123 gel-elektroforese proteinkomplekser separasjon kryssbinding multimerer SIDE
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