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Isolation of Circulating Tumor Cells in an Orthotopic Mouse Model of Colorectal Cancer

Article DOI: 10.3791/55357-v • 06:19 min • July 18th, 2017
July 18th, 2017 •
1, 1, 1, 1, 1,2,3, 1, 1,2,3
1Department of Gastrointestinal, Thoracic and Vascular Surgery, Medizinische Fakultät Carl Gustav Carus, Technische Universität Dresden, 2German Cancer Consortium (DKTK), 3German Cancer Research Center (DKFZ)

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We describe the establishment of orthotopic colorectal tumors via injection of tumor cells or organoids into the cecum of mice and the subsequent isolation of circulating tumor cells (CTCs) from this model.

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Isolation Circulating Tumor Cells Orthotopic Mouse Model Colorectal Cancer Microsurgical Technique Injecting Tumor Cells Cecal Wall Reproducible Tumors Metastatic Colorectal Tumors Circulating Tumor Cell Biology Atraumatic Forceps Immunocompromised Mouse Intra-cecal Injection One Milliliter Syringe 30 Gauge Canella Micro Injection Pump Micro Manipulator Binocular Surgical Microscope Stretching Tissue Serosa
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