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Journal / Neuroscience / Rekombinant α-β og den ble Synucleins stimulere…
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Neuroscience
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JoVE Journal Neuroscience
Rekombinant α-β og den ble Synucleins stimulere Protein fosfatase 2A katalytisk delenhet aktivitet i cellen gratis analyser
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Rekombinant α-β og den ble Synucleins stimulere Protein fosfatase 2A katalytisk delenhet aktivitet i cellen gratis analyser

Article DOI: 10.3791/55361-v • 09:36 min • August 13th, 2017
August 13th, 2017 •
1, 1, 1, 1, 1, 1
1Department of Biomedical Sciences, Center of Emphasis in Neurosciences, Graduate School of Biomedical Sciences, Paul L. Foster School of Medicine, Texas Tech University Health Sciences Center El Paso

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Denne publikasjonen presenterer en protokoll viser hvordan måle aktiviteten av rekombinant protein fosfatase 2A katalytisk delenhet (PP2Ac) i rekombinant α-synuclein, β-synuclein eller γ-synuclein proteiner ved hjelp av en enkel kolorimetrisk analysen. Vi viser funn om α-synuclein spesifisitet mot PP2Ac i musen hjernen.

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Nevrovitenskap problemet 126 kolorimetrisk analysen dephosphorylation malakitt fosfat fosfatase aktivitet PP2A katalytisk delenhet rekombinante proteiner synucleins threonin phosphopeptide
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