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Journal / Neuroscience / Recombinante α-β e γ-Synucleins estimular a…
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Neuroscience
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JoVE Journal Neuroscience
Recombinante α-β e γ-Synucleins estimular a proteína fosfatase 2A subunidade catalítica atividade ensaios em células grátis
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Recombinante α-β e γ-Synucleins estimular a proteína fosfatase 2A subunidade catalítica atividade ensaios em células grátis

Article DOI: 10.3791/55361-v • 09:36 min • August 13th, 2017
August 13th, 2017 •
1, 1, 1, 1, 1, 1
1Department of Biomedical Sciences, Center of Emphasis in Neurosciences, Graduate School of Biomedical Sciences, Paul L. Foster School of Medicine, Texas Tech University Health Sciences Center El Paso

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Esta publicação apresenta um protocolo mostrando como medir a atividade da proteína recombinante fosfatase 2A catalítico o subunit (PP2Ac) em resposta a recombinante synuclein-α, β-synuclein ou proteínas γ-synuclein usando um simples ensaio colorimetric. Mostramos a conclusões a respeito da especificidade do α-synuclein para PP2Ac no cérebro de rato.

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Neurociência edição 126 ensaio Colorimetric desfosforilação malaquita fosfato atividade da fosfatase PP2A subunidade catalítica proteínas recombinantes synucleins Fosfopeptida de treonina
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