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Journal / Neuroscience / El aislamiento magnético rápida y refinado CD11b…
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Neuroscience

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JoVE Journal Neuroscience
El aislamiento magnético rápida y refinado CD11b de la microglia primaria con mayor pureza y versatilidad
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El aislamiento magnético rápida y refinado CD11b de la microglia primaria con mayor pureza y versatilidad

Article DOI: 10.3791/55364-v • 07:54 min • April 13th, 2017
April 13th, 2017 •
*1, *1, 1, 1, 1, 1, 1, 1
1Biomedical Sciences & Iowa Center for Advanced Neurotoxicology, Iowa State University
* These authors contributed equally

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Aquí, se presenta un protocolo para aislar microglia de crías de ratón postnatal (día 1) para la experimentación in vitro. Este método improvisada de aislamiento genera tanto alto rendimiento y pureza, una ventaja significativa sobre métodos alternativos que permite amplia experimentación gama para los fines de elucidar la biología microglial.

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Neuroscience CD11b microglia cultivo primario aislamiento magnético el cerebro el envejecimiento neurodegeneración la neuroinflamación trastornos neurodegenerativos
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