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JoVE Journal Genetics
Sekventiel Salt ekstraktioner til analyse af Bulk kromatin bindende egenskaber af kromatin ændre komplekser
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Sekventiel Salt ekstraktioner til analyse af Bulk kromatin bindende egenskaber af kromatin ændre komplekser

Article DOI: 10.3791/55369-v • 07:41 min • October 2nd, 2017
October 2nd, 2017 •
1, 1, 1
1Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University

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Sekventiel salt udvinding af kromatin bundet proteiner er et nyttigt værktøj for fastsættelsen af de bindende egenskaber af store proteinkomplekser. Denne metode kan anvendes til at vurdere individuelle underenheder eller domæner i det samlede affinitet af et proteinkompleks til bulk kromatin rolle.

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Genetik spørgsmålet 128 kromatin sekventiel salt udvinding kromatin remodeling komplekser bindende profiler Epigenetik Histon mærker Histon anerkendelse domæner SWI/SNF Polybromo-1
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