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Genetics

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JoVE Journal Genetics
Steuerbare Ionenkanal-Expression durch induzierbare Transiente Transfektion
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Steuerbare Ionenkanal-Expression durch induzierbare Transiente Transfektion

Article DOI: 10.3791/55370-v • 10:00 min • February 17th, 2017
February 17th, 2017 •
*1, *1, 1
1The Institute for Drug Research (IDR), School of Pharmacy, Faculty of Medicine, The Hebrew University of Jerusalem (HUJI)
* These authors contributed equally

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Studieren Ionenkanäle durch ein heterolog exprimierenden System hat eine Kerntechnik in der biomedizinischen Forschung. In diesem Manuskript stellen wir eine zeiteffiziente Methode streng kontrollierten Ionenkanal-Expression durch Durchführung transienter Transfektion unter der Kontrolle eines induzierbaren Promotors zu erzielen.

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Genetik Ausgabe 120 Transfektion HEK293 Tet-Repressor ein Kanal ganze Zelle Elektrophysiologie Proteinexpression induzierbare Expression heterologe Expression Kalzium-Imaging
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