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Kontrollerbar Ion Channel Expression gjennom induserbar Transient Transfeksjon
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Kontrollerbar Ion Channel Expression gjennom induserbar Transient Transfeksjon

Article DOI: 10.3791/55370-v • 10:00 min • February 17th, 2017
February 17th, 2017 •
*1, *1, 1
1The Institute for Drug Research (IDR), School of Pharmacy, Faculty of Medicine, The Hebrew University of Jerusalem (HUJI)
* These authors contributed equally

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Studerer ionekanaler gjennom et heterologt uttrykke systemet har blitt en sentral teknikk i biomedisinsk forskning. I dette manuskriptet presenterer vi en tidseffektiv metode for å oppnå kontrollert ionekanal ekspresjon ved å utføre transient transfeksjon under kontroll av en induserbar promoter.

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Genetikk transfeksjon HEK293 TET repressor enkelt kanal hel celle elektrofysiologi protein expression induserbar uttrykk heterologe uttrykk kalsium bildebehandling
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