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Journal / Immunology and Infection / Strömlinjeformad enda Cell TCR isolering och…
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Immunology and Infection
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JoVE Journal Immunology and Infection
Strömlinjeformad enda Cell TCR isolering och generering av retrovirala vektorer för In Vitro och In Vivo uttryck för mänskliga TCRs
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Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells
Journal (Immunology and Infection)
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Strömlinjeformad enda Cell TCR isolering och generering av retrovirala vektorer för In Vitro och In Vivo uttryck för mänskliga TCRs

Article DOI: 10.3791/55379-v • 11:21 min • September 10th, 2017
September 10th, 2017 •
1, 2, 1, 1, 3, 2, 4, 1, 1
1Department of Pediatrics, Section of Diabetes and Endocrinology, McNair Medical Institute, Baylor College of Medicine, Texas Children's Hospital, 2Benaroya Research Institute at Virginia Mason, 3Center for Human Immunobiology, Baylor College of Medicine, Texas Children's Hospital, 4Department of Pediatrics, Section of Diabetes and Endocrinology, Baylor College of Medicine, Texas Children's Hospital

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De nuvarande protokollet kombinerar enda cell ihop mänskliga TCR alfa och beta kedja sekvensering med strömlinjeformad generation av retrovirala vektorer kompatibel med in vitro- och in-vivo TCR uttryck.

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Immunologi problemet 127 T-cell T cell receptorn TCR PCR sekvensering retrovirala enda cell mänskliga in vivo, in vitro- TCR uttryck
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