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Journal / Genetics / リボソーム DNA に基づいて RNA サンプルの DNA…
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Genetics

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JoVE Journal Genetics
リボソーム DNA に基づいて RNA サンプルの DNA 汚染の評価
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リボソーム DNA に基づいて RNA サンプルの DNA 汚染の評価

Article DOI: 10.3791/55451-v • 13:16 min • January 22nd, 2018
January 22nd, 2018 •
1, 1, 2, 3, 4
1Genetic and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari Agricultural Sciences and Natural Resources University (SANRU), 2Department of Industrial and Environmental Biotechnology, National Institute of Genetics Engineering and Biotechnology (NIGEB), 3Department of Plant Breeding and Biotechnology, Gorgan University of Agricultural Sciences & Natural Resources, 4RG Abiotic Stress Genomics/ RG Heterosis, Department Molecular Genetics, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)

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ここでは、RNA サンプルでゲノム DNA (gDNA) 汚染をトレースするためのプロトコルを提案する.提案手法では、リボソーム DNA (rDNA) 遺伝子の内部転写スペーサー領域 (ITS) に特異的なプライマーを利用しています。メソッドは、ほとんどの真核生物と原核生物の DNA 汚染の信頼性と機密性の高い検出に適しています。

Tags

遺伝学、問題 131、qPCR、RT qPCR、遺伝子発現解析、DNA 汚染分析、リボソーム DNA (rDNA)、リボソーム RNA (rRNA)、イネ科、原核生物、真核生物、普遍的なプライマー
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