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JoVE Journal Bioengineering
Geautomatiseerde Contractie Analyse van Human Engineered hartweefsel voor Cardiac Drug Safety Screening
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Geautomatiseerde Contractie Analyse van Human Engineered hartweefsel voor Cardiac Drug Safety Screening

Article DOI: 10.3791/55461-v • 10:39 min • April 15th, 2017
April 15th, 2017 •
1, 1, 1, 1, 1, 1, 1
1Department of Experimental Pharmacology and Toxicology, Cardiovascular Research Center, University Medical Center Hamburg-Eppendorf and DZHK (German Center for Cardiovascular Research)

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Hier tonen we de generering van humane gemanipuleerde hartweefsel van geïnduceerde pluripotente stamcellen (iPSC) afgeleide cardiomyocyten. We presenteren een methode om contractiekracht en voorbeeldige verandering van contractie patroon geanalyseerd door hERG kanaal remmer E-4031. Deze werkwijze vertoont een hoge mate van robuustheid en geschiktheid voor cardiale geneesmiddelscreening.

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Bioengineering Engineered hartweefsel hartspiercellen tissue engineering cardiale differentiatie veiligheid van het geneesmiddel farmacologie
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