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Gebruik makend van pHluorin-gelabeld receptoren Monitor subcellulaire lokalisatie en handel
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Gebruik makend van pHluorin-gelabeld receptoren Monitor subcellulaire lokalisatie en handel

Article DOI: 10.3791/55466-v • 09:59 min • March 16th, 2017
March 16th, 2017 •
1, 2, 1
1Department of Chemistry, University of Kentucky, 2Department of Biomedical Sciences, Marshall University

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Labelen van de extracellulaire domein van een membraaneiwit met een pH-gevoelige fluorofoor, superecliptic pHluorin (SEP), maakt lokalisatie, expressie en mensenhandel worden bepaald. Imaging september-gemerkte eiwitten met totale interne reflectie fluorescentie microscopie (TIRFM) maakt de kwantificering van eiwitniveaus in de perifere ER en plasmamembraan.

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Biofysica Superecliptic pHluorin receptor membraan mensenhandel lokalisatie plasmamembraan inbrengen totale interne reflectie fluorescentiemicroscopie membraaneiwit opregulatie
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