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JoVE Journal
Biochemistry

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一个定制HPLC纯化协议,产量高纯度淀粉样蛋白β42和贝塔淀粉样蛋白肽40,有能力寡聚物形成的
 
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一个定制HPLC纯化协议,产量高纯度淀粉样蛋白β42和贝塔淀粉样蛋白肽40,有能力寡聚物形成的

Article DOI: 10.3791/55482-v 06:34 min March 27th, 2017
March 27th, 2017

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在此,我们报告说,得到高纯度的β淀粉样蛋白42(Aβ42)和β淀粉样蛋白40(Aβ40)肽,能够形成低聚物量身定制的HPLC纯化协议。 β淀粉样蛋白在阿尔茨海默氏症牵连高度易聚集,疏水肽。该肽的淀粉样蛋白性质使得其净化是一个挑战。

Tags

生物化学,第121,淀粉样β,肽纯化,高效液相色谱法,阿尔茨海默氏病,低聚物形成,PICUP
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