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JoVE Journal
Biochemistry

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对酶活性G4 Resolvase1净化用G-四DNA亲和方法
 
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对酶活性G4 Resolvase1净化用G-四DNA亲和方法

Article DOI: 10.3791/55496-v 11:25 min March 18th, 2017
March 18th, 2017

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Summary

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Click here for the English version.

G4 Resolvase1结合G-四(G4)的结构与一个G4结合蛋白的紧密报道的亲和力,并表示在HeLa细胞中的大多数G4-DNA解旋活动。我们描述了线束的亲和力和G4-Resolvase1的ATP依赖的平仓活动,特别是净化催化活性的重组G4R1一个新的协议。

Tags

生物化学,121期,G4 Resolvase1,
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