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Journal / Biochemistry / Un enfoque ADN afinidad G-cuádruplex para la…
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Biochemistry

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JoVE Journal Biochemistry
Un enfoque ADN afinidad G-cuádruplex para la purificación de enzimáticamente activa G4 Resolvase1
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Un enfoque ADN afinidad G-cuádruplex para la purificación de enzimáticamente activa G4 Resolvase1

Article DOI: 10.3791/55496-v • 11:25 min • March 18th, 2017
March 18th, 2017 •
*1, *2, 3, 4, 1, 3
1Department of Cancer Biology, Wake Forest School of Medicine, 2YX Genomics, 3Department of Biology, Ball State University, 4Department of Hematology and Oncology, Roper St. Francis Hospital
* These authors contributed equally

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G4 Resolvase1 se une a estructuras de G-quadruplex (G4) con la afinidad reportado más apretado para una proteína de unión-G4 y representa la mayoría de la G4-ADN anulación actividad en las células HeLa. Se describe un nuevo protocolo que aprovecha la afinidad y anulación actividad dependiente de ATP de G4-Resolvase1 para purificar específicamente G4R1 recombinante catalíticamente activo.

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Bioquímica No. 121 G4 Resolvase1, G-cuádruplex elución dependiente de ATP la proteína G-quadruplex purificación de afinidad helicasa de ADN ARN helicasa
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