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Journal / Biochemistry / Het oplossen van Affiniteit gezuiverde eiwit…
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Biochemistry

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JoVE Journal Biochemistry
Het oplossen van Affiniteit gezuiverde eiwit complexen door Blue inheemse PAGE en Protein Correlatie Profiling
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Het oplossen van Affiniteit gezuiverde eiwit complexen door Blue inheemse PAGE en Protein Correlatie Profiling

Article DOI: 10.3791/55498-v • 09:35 min • April 1st, 2017
April 1st, 2017 •
1, 1, 1, 1
1Proteomic Mass Spectrometry, Wellcome Trust Sanger Institute

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Hier presenteren we protocollen voor affiniteitszuivering van eiwitcomplexen en hun scheiding door blauwe natieve PAGE, gevolgd door proteïne correlatie profilering middels label vrije kwantitatieve massaspectrometrie. Deze methode is nuttig voor interactomen in verschillende proteïnecomplexen lossen.

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Biochemistry Blue inwoner PAGE proteïne correlatie profilering multi-eiwitcomplex affiniteitszuivering kwantitatieve massaspectrometrie interactoom AP-MS eiwit interacties
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