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JoVE Journal Genetics
Detectie van microRNA-expressie in peritoneale membraan van ratten met behulp van kwantitatieve real-time PCR
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Detectie van microRNA-expressie in peritoneale membraan van ratten met behulp van kwantitatieve real-time PCR

Article DOI: 10.3791/55505-v • 08:56 min • June 27th, 2017
June 27th, 2017 •
1, 2, 2, 2, 2, 2, 3, 1
1Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, 2Division of Nephrology, Department of Internal Medicine, Jichi Medical University, 3Department of Medical Physiology, Meiji Pharmaceutical University

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Hier presenteren we een protocol voor de detectie van microRNA expressie in het peritoneale membraan van de ratten met behulp van kwantitatieve real-time reverse-transcriptie-polymerase kettingreactie. Deze methode is geschikt voor het bestuderen van het microRNA-expressieprofiel in het peritoneale membraan in verschillende pathologische omstandigheden.

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Moleculaire Biologie Uitgave 124 MicroRNA Rat Peritoneaal Membraan Fibrose Zuivering Opsporing qRT-PCR
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