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JoVE Journal Genetics
Påvisning af mikroRNA ekspression i peritoneal membran af rotter ved hjælp af kvantitativ real-time PCR
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Påvisning af mikroRNA ekspression i peritoneal membran af rotter ved hjælp af kvantitativ real-time PCR

Article DOI: 10.3791/55505-v • 08:56 min • June 27th, 2017
June 27th, 2017 •
1, 2, 2, 2, 2, 2, 3, 1
1Division of Nephrology, First Department of Integrated Medicine, Saitama Medical Center, Jichi Medical University, 2Division of Nephrology, Department of Internal Medicine, Jichi Medical University, 3Department of Medical Physiology, Meiji Pharmaceutical University

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Her præsenterer vi en protokol til påvisning af mikroRNA-ekspression i rotte-peritoneal membran ved anvendelse af kvantitativ revers-transkriptionspolymerase-kædereaktion i realtid. Denne metode er egnet til at studere mikroRNA ekspressionsprofilen i rotte peritoneal membran i flere patologiske tilstande.

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Molekylærbiologi udgave 124 MicroRNA rotte peritoneal membran fibrose oprensning detektion qRT-PCR
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