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Journal / Developmental Biology / Un metodo semplice per identificare le cinasi che …
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Developmental Biology

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JoVE Journal Developmental Biology
Un metodo semplice per identificare le cinasi che regolano la pluripotenza delle cellule staminali embrionali mediante screening di inibitori ad alta percentuale
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Journal (Developmental Biology)
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Un metodo semplice per identificare le cinasi che regolano la pluripotenza delle cellule staminali embrionali mediante screening di inibitori ad alta percentuale

Article DOI: 10.3791/55515-v • 07:18 min • May 12th, 2017
May 12th, 2017 •
1, 2,3, 1
1The MRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, University of Dundee, 2Department of Cancer Biology, Dana-Farber Cancer Institute, 3Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School

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Qui presentiamo un protocollo quantitativo e scalabile per eseguire schermate di piccole molecole mirate per i regolatori di chinasi della transizione pluripotente con primato naïve.

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Biologia dello sviluppo numero 123 proteina cinasi cellule staminali embrionali pluripotenza transizione naïve-primata inibitori della chinasi schermo ad alta potenza
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