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Journal / Developmental Biology / 在肉瘤细胞间质上皮转变的诱导
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JoVE Journal Developmental Biology
在肉瘤细胞间质上皮转变的诱导
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在肉瘤细胞间质上皮转变的诱导

Article DOI: 10.3791/55520-v • 11:42 min • April 7th, 2017
April 7th, 2017 •
1, 1, 1, 1, 2, 3, 4, 1, 1, 5, 2, 4, 1
1Department of Medicine, Duke University, 2Department of Bioengineering, Rice University, 3Department of Molecular Genetics and Microbiology, Duke University, 4Solid Tumor Program and the Duke Prostate Center, Duke University Medical Center, 5Duke University Medical Center

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我们在这里提出用于诱导基于微小RNA-200家族成员及grainyhead样2（GRHL2）的合并的异位表达肉瘤细胞间质上皮转换（MET）的细胞培养方法。这种方法适用于更好地理解可塑性的癌症侵袭性和治疗方法的生物学影响。

Tags

发育生物学，第122，间充质 - 上皮转变，肉瘤，转染，微RNA-200家族，GRHL2，细胞培养，上皮可塑性
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