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Journal / Developmental Biology / 肉腫細胞における間葉上皮移行の誘導…
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Developmental Biology

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JoVE Journal Developmental Biology
肉腫細胞における間葉上皮移行の誘導
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肉腫細胞における間葉上皮移行の誘導

Article DOI: 10.3791/55520-v • 11:42 min • April 7th, 2017
April 7th, 2017 •
1, 1, 1, 1, 2, 3, 4, 1, 1, 5, 2, 4, 1
1Department of Medicine, Duke University, 2Department of Bioengineering, Rice University, 3Department of Molecular Genetics and Microbiology, Duke University, 4Solid Tumor Program and the Duke Prostate Center, Duke University Medical Center, 5Duke University Medical Center

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ここでは、マイクロRNA-200ファミリーメンバーとgrainyhead様2（GRHL2）の合成異所性発現に基づく肉腫細胞における間葉上皮移行（MET）を誘導する細胞培養方法を提示します。この方法は、癌の悪性度や治療上の表現型可塑性の生物学的影響をよりよく理解するために適しています。

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発生生物学、問題122、間葉上皮移行し、肉腫、トランスフェクション、マイクロRNA-200家族、GRHL2、細胞培養、上皮可塑性
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