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Developmental Biology

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JoVE Journal Developmental Biology
Induksjon av Mesenchymale-Epitelial Transitions i sarkomceller
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Induksjon av Mesenchymale-Epitelial Transitions i sarkomceller

Article DOI: 10.3791/55520-v • 11:42 min • April 7th, 2017
April 7th, 2017 •
1, 1, 1, 1, 2, 3, 4, 1, 1, 5, 2, 4, 1
1Department of Medicine, Duke University, 2Department of Bioengineering, Rice University, 3Department of Molecular Genetics and Microbiology, Duke University, 4Solid Tumor Program and the Duke Prostate Center, Duke University Medical Center, 5Duke University Medical Center

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Vi presenterer her en cellekultur metode for indusering av mesenchymale-epitelial overganger (MET) i sarcom-celler basert på kombinerte ektopisk ekspresjon av mikroRNA-200 familiemedlemmer og grainyhead liknende 2 (GRHL2). Denne metoden er egnet for bedre å forstå den biologiske effekten av fenotypisk plastisitet på kreft aggressivitet og behandlinger.

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Developmental Biology utgave 122 Mesenchymale-epitelial overganger sarkom transfeksjon mikroRNA-200-familien GRHL2 cellekultur epitelial plastisitet
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