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Journal / Genetics / Single Molecule Analyse af Laser Lokaliserede…
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JoVE Journal Genetics
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Single Molecule Analyse af Laser Lokaliserede Psoralen Adducts

Article DOI: 10.3791/55541-v • 11:46 min • April 20th, 2017
April 20th, 2017 •
1, 1, 1, 1, 1, 1, 1
1Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health

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Lasere anvendes ofte i studier af det cellulære respons på DNA beskadigelse. Men de generere læsioner, hvis afstand, hyppighed, og kollisioner med replikationsgafler sjældent karakteriseret. Her beskriver vi en fremgangsmåde, der muliggør bestemmelse af disse parametre med laser lokaliseret interstrengstværbindinger.

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Genetik DNA damage laser localization DNA fiber interstrengstværbinding enkelt molekyle billeddannelse
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