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Journal / Immunology and Infection / 体外方法比较标的结合和CDC诱导治疗性抗体之间：应用在Biosimilarity分析…
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Immunology and Infection

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JoVE Journal Immunology and Infection
体外方法比较标的结合和CDC诱导治疗性抗体之间：应用在Biosimilarity分析
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体外方法比较标的结合和CDC诱导治疗性抗体之间：应用在Biosimilarity分析

Article DOI: 10.3791/55542-v • 07:25 min • May 4th, 2017
May 4th, 2017 •
1,2, 1, 3, 4,5, 6
1Unit for Development and Research in Bioprocesses Unit (UDIBI), National School of Biological Sciences, National Polytechnic Institute (IPN), University of Mexico (UNAM), 2School of Chemistry, National Autonomous University of Mexico (UNAM), 3Graduate Program in Chemical Sciences, National Autonomous University of Mexico (UNAM), 4Unit for Development Research and Medical Innovation in Biotechnology (UDIMEB), National School of Biological Sciences, National Polytechnic Institute (IPN), 5Department of Immunology, National Scool of Biological Sciences, National Polytechnic Institute (IPN), 6Department of Pharmacology and Unit of Translational Biomedicine (CMN 20 de noviembre), School of Medicine, National Autonomous University of Mexico (UNAM)

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该协议描述了利妥昔单抗的两个关键功能特征的体外比较：靶结合和补体依赖性细胞毒性（CDC）诱导。该方法用于参考利妥昔单抗和利妥昔单抗生物仿制药之间的对侧比较。这些测定可以在生物仿制发展过程中使用或作为其生产中的质量控制。

Tags

免疫学，第123，利妥昔单抗，生物仿制药，抗CD20，治疗性mAb，CDC，流式细胞术，Daudi细胞
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