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Immunology and Infection

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JoVE Journal Immunology and Infection
Metodi in vitro per il confronto obiettivo vincolante e CDC induzione Tra terapeutiche Anticorpi
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Metodi in vitro per il confronto obiettivo vincolante e CDC induzione Tra terapeutiche Anticorpi: Applicazioni nel Analisi Biosimilarity

Article DOI: 10.3791/55542-v • 07:25 min • May 4th, 2017
May 4th, 2017 •
1,2, 1, 3, 4,5, 6
1Unit for Development and Research in Bioprocesses Unit (UDIBI), National School of Biological Sciences, National Polytechnic Institute (IPN), University of Mexico (UNAM), 2School of Chemistry, National Autonomous University of Mexico (UNAM), 3Graduate Program in Chemical Sciences, National Autonomous University of Mexico (UNAM), 4Unit for Development Research and Medical Innovation in Biotechnology (UDIMEB), National School of Biological Sciences, National Polytechnic Institute (IPN), 5Department of Immunology, National Scool of Biological Sciences, National Polytechnic Institute (IPN), 6Department of Pharmacology and Unit of Translational Biomedicine (CMN 20 de noviembre), School of Medicine, National Autonomous University of Mexico (UNAM)

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Questo protocollo descrive il confronto in vitro di due funzionalità funzionali chiave di rituximab: indotta da bersaglio e da induzione a dipendenza della citotossicità (CDC). I metodi sono stati impiegati per un confronto tra rituximab di riferimento e biosimilar di rituximab. Questi test possono essere impiegati durante lo sviluppo biosimile o come controllo di qualità nella loro produzione.

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Immunologia Rituximab biosimilare anti-CD20 mAb terapeutico CDC citometria a flusso le cellule Daudi
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