Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Biochemistry

A subscription to JoVE is required to view this content.

Measuring G-protein-coupled Receptor Signaling via Radio-labeled GTP Binding
 

Measuring G-protein-coupled Receptor Signaling via Radio-labeled GTP Binding

Article DOI: 10.3791/55561-v 10:13 min June 9th, 2017
June 9th, 2017

Chapters

Summary

Guanosine triphosphate (GTP) binding is one of the earliest events in G-Protein-Coupled Receptor (GPCR) activation. This protocol describes how to pharmacologically characterize specific GPCR-ligand interactions by monitoring the binding of the radio-labeled GTP analog, [35S]guanosine-5'-O-(3-thio)triphosphate ([35S]GTPγS), in response to a ligand of interest.

Tags

G-protein-coupled Receptor Signaling Radio-labeled GTP Binding Pharmacology Biochemistry Cellular Membranes Functional GTP Binding Assay Plate Cells Transfection Incubation Rinse Cells Buffer 1 Cell Scraper Micro Centrifuge Tube Lysates Thaw
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter