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Journal / Biochemistry / Medición de la señalización del receptor acoplado …
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Biochemistry

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JoVE Journal Biochemistry
Medición de la señalización del receptor acoplado a la proteína G mediante unión de GTP marcada con radio
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Medición de la señalización del receptor acoplado a la proteína G mediante unión de GTP marcada con radio

Article DOI: 10.3791/55561-v • 10:13 min • June 9th, 2017
June 9th, 2017 •
*1, *1, 1, 1,2, 1,3,4
1The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, 2Department of Neurology and neurosurgery, Johns Hopkins University School of Medicine, 3Departments of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, 4Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine
* These authors contributed equally

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La unión a trifosfato de guanosina (GTP) es uno de los primeros eventos en la activación de receptores acoplados a proteína G (GPCR). Este protocolo describe cómo caracterizar farmacológicamente las interacciones específicas de GPCR-ligando mediante la monitorización de la unión del análogo de GTP marcado con radio, [35S] guanosina-5'-O- (3-tio) trifosfato ([35S] GTPγS), en Respuesta a un ligando de interés.

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Bioquímica Número 124 Fraccionamiento celular preparación de membranas GPCR agonista antagonista unión a GTPγS farmacología receptor μ-opioide
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