Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Måling af G-proteinkoblet receptorreceptor via…
JoVE Journal
Biochemistry

A subscription to JoVE is required to view this content.

JoVE Journal Biochemistry
Måling af G-proteinkoblet receptorreceptor via radio-mærket GTP-binding
Deciphering the Structural Effects of Activating EGFR Somatic Mutations with Molecular Dynamics Simulation
Journal (Biochemistry)
Deciphering the Structural Effects of Activating EGFR Somatic Mutations with Molecular Dynamics Simulation
Affinity Purification of a Fibrinolytic Enzyme from Sipunculus nudus (Video) | JoVE
Journal (Biochemistry)
Affinity Purification of a Fibrinolytic Enzyme from Sipunculus nudus (Video) | JoVE
 
Click here for the English version

Måling af G-proteinkoblet receptorreceptor via radio-mærket GTP-binding

Article DOI: 10.3791/55561-v • 10:13 min • June 9th, 2017
June 9th, 2017 •
*1, *1, 1, 1,2, 1,3,4
1The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, 2Department of Neurology and neurosurgery, Johns Hopkins University School of Medicine, 3Departments of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, 4Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine
* These authors contributed equally

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Guanosintriphosphat (GTP) -binding er en af ​​de tidligste begivenheder i G-protein-koblet receptor (GPCR) -aktivering. Denne protokol beskriver, hvordan man farmakologisk karakteriserer specifikke GPCR-ligandinteraktioner ved at overvåge bindingen af ​​den radiomærkede GTP-analog, [35S] guanosin-5'-O- (3-thio) triphosphat ([35S] GTPγS), i Svar på en ligand af interesse.

Tags

Biochemistry Cellular fractionation membrane preparation GPCR agonist antagonist GTPγS binding pharmacology μ-opioid receptor
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter