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Journal / Biochemistry / 通过放射性标记的GTP结合测量G蛋白偶联的受体信号…
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Biochemistry

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JoVE Journal Biochemistry
通过放射性标记的GTP结合测量G蛋白偶联的受体信号
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Journal (Biochemistry)
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Journal (Biochemistry)
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通过放射性标记的GTP结合测量G蛋白偶联的受体信号

Article DOI: 10.3791/55561-v • 10:13 min • June 9th, 2017
June 9th, 2017 •
*1, *1, 1, 1,2, 1,3,4
1The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, 2Department of Neurology and neurosurgery, Johns Hopkins University School of Medicine, 3Departments of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, 4Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine
* These authors contributed equally

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Summary
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三磷酸鸟苷（GTP）结合是G蛋白偶联受体（GPCR）激活中最早的事件之一。该方案描述了如何通过监测放射性标记的GTP类似物[ 35 S]鸟苷-5'-O-（3-硫代）三磷酸（[ 35 S]GTPγS）的结合在药理学上表征特异性GPCR-配体相互作用对感兴趣配体的反应。

Tags

生物化学，第124期，细胞分离，膜制备，GPCR，激动剂，拮抗剂，GTPγS结合，药理学，μ-阿片受体
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