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JoVE Journal Biochemistry
Mätning av G-proteinkopplad receptorreceptor via radiomärkt GTP-bindning
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Mätning av G-proteinkopplad receptorreceptor via radiomärkt GTP-bindning

Article DOI: 10.3791/55561-v • 10:13 min • June 9th, 2017
June 9th, 2017 •
*1, *1, 1, 1,2, 1,3,4
1The Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine, 2Department of Neurology and neurosurgery, Johns Hopkins University School of Medicine, 3Departments of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, 4Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine
* These authors contributed equally

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Guanosintrifosfat (GTP) -bindning är en av de tidigaste händelserna vid aktivering av G-proteinkopplad receptor (GPCR). Detta protokoll beskriver hur man farmakologiskt karakteriserar specifika GPCR-ligand-interaktioner genom att övervaka bindningen av den radiomärkta GTP-analogen, [35S] guanosin-5'-0- (3-tio) trifosfat ([35S] GTPyS), i Svar på en ligand av intresse.

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Biochemistry Cellfraktionering membranberedning GPCR agonist antagonist GTPyS-bindning farmakologi μ-opioidreceptor
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