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JoVE Journal Developmental Biology
Een 5-m-dot-blot-analyse die het DNA-methyleringsniveau van chondrocytedifferentiatie quantificeert
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Een 5-m-dot-blot-analyse die het DNA-methyleringsniveau van chondrocytedifferentiatie quantificeert

Article DOI: 10.3791/55565-v • 10:07 min • May 17th, 2017
May 17th, 2017 •
1,2, 3, 4,5, 2,6, 2, 2, 1,2
1Guangzhou Medical University, 2Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Laboratory of Digital Orthopeadic Engineering, Department of Orthopedics, Shenzhen Second People's Hospital (The First Hospital Affiliated to Shenzhen University), 3Department of Chemistry, The Chinese University of Hong Kong, 4School of Biomedical Engineering, Shanghai Jiao Tong University, 5Renji Hospital Clinical Stem Cell Research Center, Shanghai Jiao Tong University School of Medicine, 6Shantou University Medical College

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Wij presenteren een methode om DNA-methylering te kwantificeren op basis van de 5-methylcytosine (5-mC) dot blot. We bepalen de 5-mC niveaus tijdens chondrocyten dedifferentiatie. Deze eenvoudige techniek kan gebruikt worden om snel het chondrocytfenotype in ACI-behandeling te bepalen.

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Ontwikkelingsbiologie Uitgave 123 Celcultuur Monolayer Uitbreiding Chondrocyten dedifferentiatie DNA-methylering 5-mC dot blot analyse Genomische DNA extractie
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