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Journal / Developmental Biology / Um Ensaio de Blot de Ponto de 5 mC Quantificando…
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Developmental Biology

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JoVE Journal Developmental Biology
Um Ensaio de Blot de Ponto de 5 mC Quantificando o N�el de Metila�o de ADN da Dediferencia�o de Condrocitos
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Um Ensaio de Blot de Ponto de 5 mC Quantificando o N�el de Metila�o de ADN da Dediferencia�o de Condrocitos

Article DOI: 10.3791/55565-v • 10:07 min • May 17th, 2017
May 17th, 2017 •
1,2, 3, 4,5, 2,6, 2, 2, 1,2
1Guangzhou Medical University, 2Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Laboratory of Digital Orthopeadic Engineering, Department of Orthopedics, Shenzhen Second People's Hospital (The First Hospital Affiliated to Shenzhen University), 3Department of Chemistry, The Chinese University of Hong Kong, 4School of Biomedical Engineering, Shanghai Jiao Tong University, 5Renji Hospital Clinical Stem Cell Research Center, Shanghai Jiao Tong University School of Medicine, 6Shantou University Medical College

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Apresentamos um método para quantificar a metilação do DNA com base na mancha ponto 5-metilcitosina (5-mC). Determinamos os níveis de 5-mC durante a desdiferenciação de condrócitos. Esta técnica simples poderia ser usada para determinar rapidamente o fenótipo condrócito em tratamento com ACI.

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Biologia do Desenvolvimento Edição 123 Cultura celular expansão monocamada desdiferenciação de condrócitos metilação do DNA análise dot-blot 5-mC extração de DNA genômico
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