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JoVE Journal Developmental Biology
Un ensayo de transferencia de puntos de 5 mC cuantificando el nivel de metilación de ADN de la distredenciación de condrocitos
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Un ensayo de transferencia de puntos de 5 mC cuantificando el nivel de metilación de ADN de la distredenciación de condrocitos

Article DOI: 10.3791/55565-v • 10:07 min • May 17th, 2017
May 17th, 2017 •
1,2, 3, 4,5, 2,6, 2, 2, 1,2
1Guangzhou Medical University, 2Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Laboratory of Digital Orthopeadic Engineering, Department of Orthopedics, Shenzhen Second People's Hospital (The First Hospital Affiliated to Shenzhen University), 3Department of Chemistry, The Chinese University of Hong Kong, 4School of Biomedical Engineering, Shanghai Jiao Tong University, 5Renji Hospital Clinical Stem Cell Research Center, Shanghai Jiao Tong University School of Medicine, 6Shantou University Medical College

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Se presenta un método para cuantificar la metilación del ADN basado en la 5-mctilcitosina (5-mC) dot blot. Se determinaron los niveles de 5-mC durante la desdiferenciación de condrocitos. Esta técnica simple podría utilizarse para determinar rápidamente el fenotipo de condrocito en el tratamiento de ACI.

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Developmental Biology Edición 123 Cultivo celular expansión monocapa desdiferenciación de condrocitos metilación del ADN análisis de transferencia de puntos de 5 mC extracción de ADN genómico
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