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Journal / Developmental Biology / 5-mC Nokta-Blot Testi Kondrosit Farklılaşmasımın…
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Developmental Biology

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JoVE Journal Developmental Biology
5-mC Nokta-Blot Testi Kondrosit Farklılaşmasımın DNA Metilasyon Seviyesinin Nicelendirilmesi
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5-mC Nokta-Blot Testi Kondrosit Farklılaşmasımın DNA Metilasyon Seviyesinin Nicelendirilmesi

Article DOI: 10.3791/55565-v • 10:07 min • May 17th, 2017
May 17th, 2017 •
1,2, 3, 4,5, 2,6, 2, 2, 1,2
1Guangzhou Medical University, 2Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Laboratory of Digital Orthopeadic Engineering, Department of Orthopedics, Shenzhen Second People's Hospital (The First Hospital Affiliated to Shenzhen University), 3Department of Chemistry, The Chinese University of Hong Kong, 4School of Biomedical Engineering, Shanghai Jiao Tong University, 5Renji Hospital Clinical Stem Cell Research Center, Shanghai Jiao Tong University School of Medicine, 6Shantou University Medical College

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DNA metilasyonunu 5-metilsitozin (5-mC) nokta leke bazında ölçmek için bir yöntem sunuyoruz. Kondrosit farklılaşması sırasında 5-mC düzeylerini saptadık. Bu basit teknik ACI tedavisinde kondrosit fenotipini hızlı bir şekilde belirlemek için kullanılabilir.

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Gelişim Biyolojisi Sayı 123 Hücre kültürü tek tabaka genleşmesi kondrosit ayırımı DNA metilasyonu 5-mC nokta leke analizi genomik DNA ekstraksiyonu
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