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Journal / Biochemistry / Lipid Droplet Isolation for Kvantitativ…
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Lipid Droplet Isolation for Kvantitativ massespektrometri Analyse
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Lipid Droplet Isolation for Kvantitativ massespektrometri Analyse

Article DOI: 10.3791/55585-v • 10:23 min • April 17th, 2017
April 17th, 2017 •
1, 2, 2, 1
1Heinrich Pette Institute, Leibniz Institute for Experimental Virology, 2Core Facility Mass Spectrometric Proteomics, University Medical Center Hamburg-Eppendorf

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Lipiddråber er vigtige organeller for replikationen af ​​flere patogener, herunder hepatitis C-virus (HCV). Vi beskriver en fremgangsmåde til isolering af lipiddråber til kvantitativ massespektrometri af associerede proteiner; den kan anvendes under forskellige betingelser, såsom virusinfektion, miljømæssig stress, eller lægemiddelbehandling.

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Biokemi lipid dråbe lipidlegemer kvantitativ massespektrometri stabil isotop mærkning med aminosyrer i cellekultur (SILAC) protein kvantificering hepatitis C-virus (HCV) virusinfektion
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